Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAACPELVDATFAILASGWHSTAVEVGGRWVFKFPRGAEAEAALLREAAVLRLVRPA--VSLPVPAPQVFAG--PPLFSRHEKLPGGYLLGEDYRRLDEPARQAVGDALGRFYAELHRLPPARMRAVGAL-PVRPWESPAAMRRRALPLLPAAWRERAERLVREYAKLP--ADPLGSIFGFFDGHGWNMAFDSEAGRLNGVYDFADAGIGPLHQEFIYSA-----FIDADLTERIVAAYERFSGR-RLERRRIALL-TAAHRLSELAELADEPRHLPDMLAGALDGLALAEAQGLA 5IQI Chain:C ((23-302)) ---FDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGFAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHN--DFSCNHLLLDGN-NRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGI-KNIKQEFIENGRKEIYKRT---------

General information: TITO was launched using: RESULT: Template: 5IQI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1159 -4562 -3.94 -17.15 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain C : 0.69 3D Compatibility (PKB) : -3.94 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.424

(partial model without unconserved sides chains): PDB file : Tito_5IQI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5iqi-query.scw PDB file : Tito_Scwrl_5IQI.pdb: