Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNAHVPSGPDELTPEW-LSRALGAEVTSVSWERVGSGQIGACYRLTLKGEEKVPARLVAK-MASEDPTTRAFLA-SAYRTEVLFYRDVVPTVAVRTPR-CHYQAIADDNSAFVLL-LEDMAPAEQGDQLAG-CTPQQAAAAVT--NLAGL-HGPRWCDKTLTEIPWLSVVTEDDAAVLTEVFAPAVDAFVQRFTGRLSDEDIDTLRRVAD-RIA-AWAVAR-----PERFGLVHGDYRLDNLL-FAPPPQQTVTAVDWQTLSIGHP-VRDLSFF-------------LGTAMDPAERSAHERALVETYHTALTGYGVDDYDLQSCWE-DYRFGALQGP-LITVLGCAVGTPTERGDRMFLAMTR-RS-CAAIRELGSLELI 3DXP Chain:A ((20-359)) ------RFDTEALEAWMRQHVEGFAGPLSVEQFKGQSNPT--FKLVTPGQTYV-------MRAKPGAIEREYRVMDA-----------LAGTDVPVAKMYALCEDESVIGRAFYIMEFVSGRVLWDQSLPGMSPAERTAIYDEMNRVIAAMHTVDYQAIGLGDYGKPG-----------NYFQRQIERWTKQYKLSETES-------IPAMDSLMDWLPQHIPQEDADLTSIVHGDYRLDNL-MFHPTEPRVLAVLDWELSTLGHPMG-DFGYHCMSWHIAPGQFRGIAGLDHAALGIPDEASYRKLYEQRTGRPITGDWNFYLAFSMFRIAGILQGIMKRVVDG---TASSAQALDAGKRARPMAEMGWEYAKKAKQ---

General information: TITO was launched using: RESULT: Template: 3DXP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1341 34804 25.95 118.38 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 25.95 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.148

(partial model without unconserved sides chains): PDB file : Tito_3DXP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3dxp-query.scw PDB file : Tito_Scwrl_3DXP.pdb: