Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHGRSRLYRYLKSGRLSAKAGFDRYPD-----------LRVKQLGGSHNVYLVSDHRSHKKCIVKSFAGSGPKSVQHMEKEYKRLRQAGRLVDDRRWVHVA----RPLCKSNGEGFFAE-ELVSGKALGDYMRDAMANGRDSELYEKLTMLAGFLALLHKKTERPSHVKPSNIRDELKKHARQASKGGAFAPHELKRIESLVDNVTSNDLISQTRRGLVHGDANPSNFLYDKK-RLNVIDMERSGYRD-------------------------------------PVYDLGMLAGELCHYAMKYGGNEYKADPFIGHLYWTYAGNFKDQLGTFIRLTGR--------NPIYMANSLLRIARHPFFSPEYKRRLARKAYECLKSLKK--- 1B6C Chain:B ((14-339)) ----TTLKDLIYD-MTTSGSGSGLPLLVQRTIARTIVLQESIGKGRFGEVWRGKWRGEEVAVKIFSSRE---------ERSWFREAEIYQTVMLRHENILGFIAADNKDNGTWTQLWLVSDYHEHGSLFDYLNRYTVT--VEGMIKLALSTASGLAHLHMEIVGTQ-----------------------------------------------GKPAIAHRDLKSKNILVKKNGTCCIADLGLAVRHDSATDTIDIAPNHRVGTKRYMAPEVLDDSINMKHFESFKRADIYAMGLVFWEIARRCSIGGIHEDYQLPYYDLVPSDPSVEEMRKVVCEQKLRPNIPNRWQSCEALRVMAKIMRECWYANGAARLTALRIKKTLSQLSQQEG

General information: TITO was launched using: RESULT: Template: 1B6C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1330 18263 13.73 69.97 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.62 3D Compatibility (PKB) : 13.73 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.291

(partial model without unconserved sides chains): PDB file : Tito_1B6C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1b6c-query.scw PDB file : Tito_Scwrl_1B6C.pdb: