Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSINFSNLSKNINFINFSKISRGNNKELKYIVKDSEMNRYLFKINSIIH-LDNKVNEFRFAKRIEHL---PFEKSEIVDLCVFNQNRYICSIYKWIDGKILEDQ-IKNYPSNKQYSFGFKAGRSLKLIHDLPIRDEE---LK---QVEELPVRKIIEYYHQNKR---FENDNIIIDFILKNNSKIKDEKICFLHGHYSIKNFIIKNN-DEISLIDYNQCYFGDYVQDFSKNEIYNS-YYSEQFAQGIIDGYFYNRKIPDTFWLKYAYYAAYNCITLFLWASKQEVISTKIKIKEMIQNVLNQFNNFESIIPNWYKKNVLSKERQKFINSPYTLEKILGTPKKENKNDSKIKYFDEMYDDNDDDLLDEKMMDSFKEFLKNVAKK 5IQI Chain:C ((27-287)) ------------KVDSIEIIGSGY-DSVAYLVN----NEYIFKTKFSTNKKKGFAKEKAIYNFLNTNLETNVKIPNIEYSYIS--DELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYN-DLTDIEKDYIESFMERLNAT--TVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNI--DIEKAKEYQDIVEEYYPIETIVYGIKNIK--------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5IQI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1056 4374 4.14 17.85 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain C : 0.64 3D Compatibility (PKB) : 4.14 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.203

(partial model without unconserved sides chains): PDB file : Tito_5IQI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5iqi-query.scw PDB file : Tito_Scwrl_5IQI.pdb: