Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEMRGGDLEAAALALWPDLARQMGEEAGLWRAAPLARREDARVARVLLRLDGPEG-RRLVLKHQARPVVPDKFADGIAAHVAVQEVWPEGLPVLQAVD--LERQACVMEYLPARPLSVLLEGAPLETQAALLRRAGAWMDGFHRALHGEARVFQP--KFTIHFLRSVMGELRAGERRVAEPERFLVCAEALCAMQPDFEGQETVTAQTHGDLHQRNLVLDE-TRCWGVDFAGGRVVPVGHDIARLLTDYAILHAPKEAIPKGQVLPPQAQSAFFEGYGLVGPGDPSVQLLLRNRVLAEWWGLPAREAERSRAQARRFGRLMALVDRVFG 4GKH Chain:A ((35-266)) ----------------------------------------GQSGATIYRLYGKPNAPELFLKHGKGS-VANDVTDEMVRLNWLTA--FMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEY-PDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFD--DERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEF-------------SPSLQKRLFQKYGIDNPDMNKLQFHL--------------------------------------

General information: TITO was launched using: RESULT: Template: 4GKH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 971 -24219 -24.94 -107.16 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -24.94 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.243

(partial model without unconserved sides chains): PDB file : Tito_4GKH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4gkh-query.scw PDB file : Tito_Scwrl_4GKH.pdb: