Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVNLDAEIYEHLNKQIKINELRYLSSGDDSDTFLCNEQYVVKVPKRDSVRISQKRELELYRFLE-NCKLSYQIPAVVYQ--SDRFNIMKY--IKGERITYEQYHKLSEKEKDALAYDEATFLKELHSIEIDCSVSLFSDALVNKKDKFLQDKKLLISILEKEQLLTDEMLEHIETIYENILSNAVLFKYTPCLVHNDFSANNMIF-RNNRLFGVIDFGDFNVGDPDNDFLCLLDCSTDDFGKEFGRKVLKYYQHKAPEVAERKAELNDVYWSIDQIIYGYERKDREMLIKDVSELLQTQAEMFIF 5IQG Chain:B ((20-291)) ---------EHYFDNFKVDSIEIIGS---SVAYLVNNEYIFKTKF------GYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLD----YTDISECTIDNKQNVLEEYILLRETIYND--LTDIEKDYIESFMER-LNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEFI-----------------

General information: TITO was launched using: RESULT: Template: 5IQG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1132 17864 15.78 69.51 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain B : 0.76 3D Compatibility (PKB) : 15.78 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.431

(partial model without unconserved sides chains): PDB file : Tito_5IQG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5iqg-query.scw PDB file : Tito_Scwrl_5IQG.pdb: