Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHTHDVTVMGDVVRKRYVSWD--DREPEREWAGLVHLSRESPGLAPTPVAREVEDGRPVVVMSLVPGEPLTSA--LSAAQSKAFAEVLRRLFAVPVPEDLAERANGPQVMRDHVREWLSEDYDLTQCQ---DQALVRTAKDRAVAWLAVV--QLPDLVDRVVAQGDGNLDNVLWDGEVCRLIDWEEYGASDLTYEIADVVEHASSR---------------LTRSLDVDALLADLDL-DPT-------QRERLAVHRLLLACFWLMMLLPGNGGFKRNPAGSTEDQARHVLALLDQS 3C5I Chain:A ((67-334)) ---------TRVLFRIYGKHVDELYNTISEFEVYKTMSK-YKI-APQLLNTF----NGGRIEEWLYGDPLRIDDLKNPTILIGIANVLGKFHTLSRKRHLPEHWDRTPCIFKMMEKWKNQLFKYKNIEKYCDIHKYIKESDKFIKFMKVYSKSDNLANTIVFCHNDLQENNIINTN-CLRLIDFEYSGFNFLATDIANFFIETSIDYSVSSYPFFEIDKKKYISYENRKLFITAYLSNYLPTPKLIDEILEAVEVQALGAHLLWGFWSIIRG-------------------------

General information: TITO was launched using: RESULT: Template: 3C5I.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 809 -16645 -20.57 -74.31 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -20.57 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.276

(partial model without unconserved sides chains): PDB file : Tito_3C5I.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3c5i-query.scw PDB file : Tito_Scwrl_3C5I.pdb: