Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDELIMESLISRIKNNFPEINIKKFYAIESGWMNYIIVIN----DAIVFRFPKTRKMVKKFQKEIRLLSILHN-PPVKVPEYVFISKD--EPV----YGGYYKINGVPLNNSKS--LGKGILSDFIRFLNYLKSYNGYKFHSNDIDIYNYKTWKYYENSLIQMFSESLADHIKESYFNNIVNLMESAFSDLEPDDFSLIHGDLSNGNVIINRRHDRINGIIDWSDSCYGDMALDIAAIVDNYPIAALDPLLLNYEQKF---SHRAIKRVLFYRAVSPLYYAHYMARTGNNDLKNYCDQIINGRKYKIRMGAAEKMLKNK 5IGH Chain:A ((6-288)) --TADTSQLYALAAR-HGLKLHGPLTVNELGLDYRIVIATVDDGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTAMVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVADDVDRVRREFV--VNDKRLHRWQRWLDDDS--SWPDFSVVVHGDLYVGHVLIDNT-ERVSGMIDWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVTYALFALDSGNEE----------------------------

General information: TITO was launched using: RESULT: Template: 5IGH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1164 -26527 -22.79 -99.35 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -22.79 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.402

(partial model without unconserved sides chains): PDB file : Tito_5IGH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igh-query.scw PDB file : Tito_Scwrl_5IGH.pdb: