Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------------------KRCD-VGIYNSDGTGPRAYKNIDIPASCSQWDFDFEYGGVTYTVRLHYEGCRTEIIGRPQ-LPSHLIIR-G 1FNF Chain:? ((1236-1326)) VPPPTDLRFTNIGPDTMRVTWAPPPSIDLTNFLVRYSPVKNEEDVAELSISPSDNAVVLTNLLPGTEYVVSVSSVY------EQHESTPLRGRQKTG

General information: TITO was launched using: RESULT: Template: 1FNF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 175 2402 13.73 38.74 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : 13.73 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.361

(partial model without unconserved sides chains): PDB file : Tito_1FNF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1fnf-query.scw PDB file : Tito_Scwrl_1FNF.pdb: