Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAEQDGQKLFLKRNSNP-----------FIAALSAEGI---VPKLVWTKRIETGEVVTAQHWKNGRELSSN-----------------EMKQTRVAHLLKKIHNSRPLLSMLKRMEMEPITPEIMLNKINASLSREVLTHHIVRKSLTYLEEHIP--SLDSRFFTVVHGDVNHNNWLLSDRDE-LFLVDWEGAMIADPAIDIGMLLYNYVPQQQWSEWLETYGVQESL---NLNKRMKWYTVIQSIGLVQWYEEQKRYKDMNTWLKFLNEVMNSNMFI 5IWU Chain:A ((43-297)) -----GIDWVLRLPRREDVMPRTKVEKQALDLVNKYAISFQAPNWIIYT-----EELIAYKKLDGVPAGTIDHNIGNYIWEIDINNVPELFHKSLGRVLAELHSIPSNKAA--ALDLVVHTPEEARMSMKQRMDAVRAKFGVGENLWNRWQAWLNDDDMWPKKTGLIHGDVHAGHTMIDKDANVTGLIDWTEAKVTDVSHDFIFNYRAF-GEEGLEALILAYKEIGGYYWPKMKEHIIELNAAYPVSIAEFALVSGIEEYEQMAKEAL----------

General information: TITO was launched using: RESULT: Template: 5IWU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 905 -2781 -3.07 -12.75 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -3.07 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.397

(partial model without unconserved sides chains): PDB file : Tito_5IWU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5iwu-query.scw PDB file : Tito_Scwrl_5IWU.pdb: