Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEFQLDKDWRLQPI-KGATGQTYMGIKE-TEKVFIKRNTSP-----------LLAALSK-EGL-APKMIWTKRTANGDVL----TAQEWLEGRLLHA-----DEIGKRNDVIDVLYQLHHSNLLKDMLKKIGGEVCTPLTMLKEYKRQLPKEL----KRNSYLKEVITYLYNHLPEYPETSYVAVHGDVNHRNWLVSN--NYLYLVDWDSVMVADPAVDLGMILSHYVPRSGWNQWLLSYGLI---PNESTVQRIYWYGLFSFLQEIVRHHQEGERRAMTAEILQLKRMFSI 5IGI Chain:A ((26-298)) ---------PLTVNELGLDYRIVIATVDDGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA-VIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDA--GMLIRTPTQARQKVADDVDRVRREFVVNDKRLHRWQRWLDDDS--SWPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMAAHLMVFGE-EGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVTYALFALDSGNEEYLAAAKAQLA-----

General information: TITO was launched using: RESULT: Template: 5IGI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1026 -4222 -4.12 -17.67 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -4.12 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.303

(partial model without unconserved sides chains): PDB file : Tito_5IGI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igi-query.scw PDB file : Tito_Scwrl_5IGI.pdb: