Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQLTHESQQLTTPKENGWCGPLPRLVASLSSLVLPLPRTESYEFAGCAVNNRFDPRQGAFRDLPSSPPIPLAVSLPLNNPPANFRSSAASMPPSPVRRPAVPPRQPFTSRTNALEGFPSPHPSSTPSKRRLAPSATLPPPSYAREHGIFLGRRRPKDCEGVGQFVKRVHWEGKVMVVKYGR------SVRQAEVDVMKMVRERTHVPVPEVFGTAFDE---------AAGELFIYQEFIEGTTLTAAWPTLSHPDLSRIKSDFSRYVHELGALALPPDASLGSCDASSSYRLSEPCHAVFAQKPEPKVQSVEDFTRWIRFE-----ISRRSRDLANSRKDFDLERL-FRDGDVGLVHADLHGENILVKNG---RIAAILDWELAAWMPPLVEALSLVEYSRMFDEDAGAPIDALIDALG----LEDATRDVLDGLCTALRAWPDEKWEEVS 6EF6 Chain:A ((51-307)) ---------------------------------------------------------------------------------------------------------------------------------------------------------------LSENATYLVEDGEHQSILRVHRQDYHQPHEIESELDWLAALRTDSDVTVPTVVPARDGRRVVTVDPAD-VPRHVVHFEMVGGAEPDEES------LTLDDFQTLGRITASLHEHSQRWTRPAG-FGR-FSWDWEHCLGD------------TPRWGRWQDAEGVGASETALLTRAQ-DLLHRKLEEYGSGPDRYGLIHADLRLANLLVDSSTPQRTITVIDFDDCGFGWYFYDFGTAVSFIEHD-PRLGEWQESWVAGYRSRRELPAADEAMLPSFV---------------

General information: TITO was launched using: RESULT: Template: 6EF6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 906 5501 6.07 24.34 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 6.07 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.209

(partial model without unconserved sides chains): PDB file : Tito_6EF6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-6ef6-query.scw PDB file : Tito_Scwrl_6EF6.pdb: