Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEQVQADRMILEDLLVSIQRDLGLVVHEAISIRRGWLNLKWKLETDQGTYLLKQYNRERFKLYDEEVLLMAFSQQIRLRRRGVKCPKLLTNEGRYFLKSDQGERFMVMEFCEGQLVAPGG---TNADQMRALGREVALMHRVLNDGSLGVKDKPQFVPPSRMERLDYWKRVMEQAAESEIPEVLTALETQYTATERFRDELNPM---QPGWAHRDVWVDNLLFRPAGVAAILDFDRLNYDYPQLDIARAVLSCALHSELDVVLVSAFMEGYREEREVPKGYLVQGIRWLWYMESVWWVNANILEHQGPPARFAWEMDWLAKHLDMLPELLEQV--- 3HAM Chain:A ((1-299)) --MVNLDAEIYEHLNKQIKI------NELRYLSSGDDSDTFLCN-EQYVVKVPKRDSVRISQKREFELYRFLENC----KLSYQIPAVVY----------QSDRFNIMKYIKGERITYEQYHKLSEKEKDALAYDEATFLKELHSIEIDCSVSLFSDALVNKKDKFLQDKKLLISILEKEQLLTDEMLEHIETIYENILNNAVLFKYTPCLVHNDFSANNMIFRNNRLFGVIDFGDFNVGDPDNDFLCLL----------DCSTDDFGKEFGRKVLKYYQHKAPEVAERKAELNDVYWSIDQIIYGYERKD----REMLIKGVSELLQTQAEMFIF

General information: TITO was launched using: RESULT: Template: 3HAM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1276 -17494 -13.71 -60.32 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -13.71 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.197

(partial model without unconserved sides chains): PDB file : Tito_3HAM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ham-query.scw PDB file : Tito_Scwrl_3HAM.pdb: