Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSALDEVEKVLWDHYGLRGPFEVTALAAEGRNNSLVAFRSPGGAFVWK-QGSSALAPVQQREFALLAWLAEQDLPFRMAAPLRTLAGAWGWEGGRGLNVLLPLLPGERLPETADAWHALG-TALNALHAALARFPLEGAPRLLDWAKLGDLHPR-VPDPLALSFEQPDLWGDEATLHSWRLAFGEMLWRVRDAYAALPH-QVVHGDFNSVNVLF----GGGGVSAVLDFEFACPGPRLLDVATALSEVLVRPEPEWA-L-AQAFLRGYGQLTLQERAALPAALLLRQ-AAVGVWGLGLALEDGPNATTQARLRELGTVSAWLKTQGDRLSTG----------------------- 3CSV Chain:A ((4-333)) -SREDEIRDFLAT----------HGYADWNRTPRYQRLRSPTGAKAVLMDWSPEEGGDTQPFVDLAQYLRNLDI---------SAPEIYAEEHARGL-LLIEDLGDALFTEVINNDPAQEMPLYRAAVDLLIHLHDAQTPELARLD--PETLSEMTRLAFSEYRYAILGDAAEDNRKRFEHRFAQILSAQLE-----GDMVFVHRDFHAQNLLWLPEREGLARVGVIDFQDAKLGHRAYDLVSLLQDARRDVPAQVEAQMIDHYIQATGVDESHFRSAYAVIAVQRNMRILGIFAR-LSQRFGK----RHYIEFVPRVWAHFERGLAHPALASAAEEILNALPAPAPEVLERLRA

General information: TITO was launched using: RESULT: Template: 3CSV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1325 195 0.15 0.68 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 0.15 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.327

(partial model without unconserved sides chains): PDB file : Tito_3CSV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3csv-query.scw PDB file : Tito_Scwrl_3CSV.pdb: