Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSGGNMAEKDLATRVARVLGRN-SVRLEDLPPGSSRGAARVLSEEGEPIAFLRIDLGVATDVTVGKALVSEMALVKRAHDL-GFPVPKVFALLNDP-------PVAVMELAAGTARPDE---AEAERVGPEFMGHIARLHSIAPAEFGLD-AP----ATVSDAIRQDLDCYMADAEARGITRRPLVRLAARILRHALPRSAEPPAMLHGDVGAGNFMVDN-GRVSAILDWELAHAGDIHEDLAWLWTRGAHTAFGDPRQRIAEYEAAAGKALD--PDRLAWHVAFVTFKSVIGLQRRMYAQGDDPGLLIILIAILTYETLLCAALGRILGIQLDMMEEAPVEGVTETVRLIELMRHVAPPANREGELLQQYLRAEAAQRPWNADRLRADARERLGAHPDQLDMLVDSAAPDRFADLLRVFGAAAGRRCKAFPNAERRVRRALAIGLGKQN 5IGI Chain:A ((5-279)) ---TTADTSQLYALAAR-HGLKLHGPLTVNELGLDYRIVIATVDD-GRRWVLRIPRRAEV----SAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA-VIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVADDVDRVRREF----VVNDKRLHRWQRWLDDDSS-WPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMAAHLMVF---GEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVTYAL--------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5IGI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1106 -24743 -22.37 -97.41 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -22.37 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.235

(partial model without unconserved sides chains): PDB file : Tito_5IGI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igi-query.scw PDB file : Tito_Scwrl_5IGI.pdb: