Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDELIMESLISRIKNNFPEINIKKFYAIESGWMNYIIVIN----DAIVFRFPKTRKMVKKFQKEIRLLSILHN-PPVKVPEYVFISKD--EPV----YGGYYKINGVPLNNSKS--LGKGILSDFIRFLNYLKSYNGYKFHSNDIDIYNYKTWKYYENSLIQMFSESLADHIKESYFNNIVNLMESAFSDLEPDDFSLIHGDLSNGNVIINRRHDRINGIIDWSDSCYGDMALDIAAIVDNYPIAALDPLLLNYEQKF---SHRAIKRVLFYRAVSPLYYAHYMARTGNNDLKNYCDQIINGRKYKIRMGAAEKMLKNK 5IGI Chain:A ((6-288)) --TADTSQLYALAAR-HGLKLHGPLTVNELGLDYRIVIATVDDGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA-VIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVADDVDRVRREFV--VNDKRLHRWQRWLDDDS--SWPDFSVVVHGDLYVGHVLIDNT-ERVSGMIDWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVTYALFALDSGNEE----------------------------

General information: TITO was launched using: RESULT: Template: 5IGI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1218 -32179 -26.42 -120.97 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -26.42 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.392

(partial model without unconserved sides chains): PDB file : Tito_5IGI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igi-query.scw PDB file : Tito_Scwrl_5IGI.pdb: