Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRQAIPWRDRSFRERFERWIEANVPNATWH--V-----IKDTDLTYLLVGAL-NEERYYAKAVT-----DISRGEAGLSAFLAERHPDFVPDVIAIDSARNWLLMRDIGGEALRER-P-----------DVRRYEAALRQYAQLQRQEIDQIETYLSYGIPDRRPATLKDEIQTYLPELCAGLDRHQAEAMLALQDELLTMCDELA-TGMPMSLEHGDLHGGNIFWRERTNDLCILDWGDATVTHPFFSVRVFWNALYDLLPEEDEVAWYKQIQTMRTVYLEAWSGVAPKDVLWRHLLIAEELGCVYRALSWHVYVTRYRYDVAESSDKPAQWLNFLLEYRDLKRRFP 5UXD Chain:A ((8-253)) -----------------DALLDLAARHGLDLDGGTLRTEEIGLDFRVAFARAHDGGDWVLRLPRRPDVLERAAVEGRLLAMLAPHLDVAVPDWRIST--SELIAYPLLPGSPGLTVAADGEVSWHVDMASTVYARSLGSVVAQLHAVDAEAAAAT---GIEVRSPAQVRGAWRQDLARVGAEF--EIAPA---LRERWEAWLADDGCWPGHSVLTHGELYPAHTLVED-ERITAVLDWTTAAVGDPAKDLMFHQVSAP-----------SAIFEVALQAYAEGGG---------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5UXD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 847 -16230 -19.16 -73.77 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -19.16 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.218

(partial model without unconserved sides chains): PDB file : Tito_5UXD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5uxd-query.scw PDB file : Tito_Scwrl_5UXD.pdb: