Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MWQAVNRLLSECLGPAEIRERTELPGGDIHEAWRLSYG-EKEVFVKCNTREM-LPIFTAESDQLSLLARSKTVQVPEVYGVGSD-RDYS----FLLLEYIPLKPLDA--------HNAYCLGQQLAHLHQWSEQLQFGLDFDNDLATTPQPNSWQRRWAQFFAEKRIGWQLQLAAEKGMSFGDIDDITNAVQERLQS---HQPQPSLLHGDLWPANCAASS--NGPVIFDPA-CYWGDRECDLSMLPLY--PTLPAQIYDGYQSVWPLP-IGFIER----QSIYQLYYLLNRSNLFGGKHLINAQKAVDNLLHPEQFSL 5IGI Chain:A ((10-297)) --SQLYALAAR-HGLKLHGPLTVNELGLDYRIVIATVDDGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA-VIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISA-AVDAG--ML--IRTPTQARQKVADDVDRVRRE--------FV----VNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVTYALFALDSGNEEYLAAAKAQL-----------

General information: TITO was launched using: RESULT: Template: 5IGI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1166 -50514 -43.32 -195.03 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -43.32 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.268

(partial model without unconserved sides chains): PDB file : Tito_5IGI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igi-query.scw PDB file : Tito_Scwrl_5IGI.pdb: