Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKRKIIFFTKAPALNYGKSRLKNFLSAKDRYELSVFLIKDNLKVLEKSGYEFVVYFSGDIENLNFIDYEKIPQR-GENLGDRMFNALKAELNSNDQ----------------VILLGSDLRGITLKLIESAFSGLNNSDCVI-----APAEDGGYGLIGLRKPLDLFSNIVYSQSDVFEKTLQKATALDISI-------------ERLDTVRDIDEIIDLIKEELQTKDVEI-LGSGEYNLNYRFNKNFVCRINLGSQLHLGDEQITYE----YNALKTLESSG---VTPKPHYLKK--NSTY-IRKGFLVMDY---LVGRPLNYDTDMSIAAYLLSRVHNLEFKDTDLIRVEQPFKAMFEECFEMYSVYKN---SKIYNNSIGKYIEHFFDYVKELGIKDNISNPCIINTELNN-----RNFIINGN----NSYIIDWEKPLIGEAEQDLAHFLVPTTTNWKTDKILSDSEIEKFIYKYEKYRKINRQKLKKYFAFNVLRGI-------TWCSMAKVEYEASERILSNQDTYEKINKFLSEDYLKMLHKRFYEV 5FI9 Chain:A ((9-536)) -KLNLTCPACKVLFTALNHGLKKEPNVARVGSVAIKICKMLNIAPLDVCQSAVHLFEDDVVEVWTRSVLSPSEACGLLLGSSCFSTWNISLPSVPKPPPKPPSPPAPGAPVSRVLFLTDLH-WDHEYLEGTDPYCADPLCCRRGSGWPPNSQKGAGFWGEYSKCDLP----LRTLESLLKGLGPAGPFEMVYWTGDIPAHDVWQQSRQDQLRALTTITDLVRKFLGPVPVYPAVGNHESTPVNGFPPPFI-KGNQSSQWLYEAMAKAWEPWLPADALHTLRIGGFYALTPRPGLRLISLNMNFCSRENFWLLINSTDPAGQLQWLVEELQAAENRGDKVHII-----GHIPPGHCLKSWSWNYYKIIARYENTLAGQFFGHTHVDEFEIFYDE-ETLSRPLAVAFLAPSATTFINLNPGYRVYQIDGNYPGSSHVVLDHETYILNLTQANAAGGTPSWKRLYRARETYGLPDAMPASWHNLVYRMRDDEQLFQTFWFLYHKGHPPSEPCGTPCRLATLCAQLSAR----ADSPALCRHLM---------------

General information: TITO was launched using: RESULT: Template: 5FI9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2489 11781 4.73 25.89 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 4.73 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.146

(partial model without unconserved sides chains): PDB file : Tito_5FI9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5fi9-query.scw PDB file : Tito_Scwrl_5FI9.pdb: