Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAVYTKITQQDIDYLFQDYD-G--IEQIKGIAEGVENTNYLVNTKNQNKFIFTIFEKRTKRSDLPFFHKAMSEFYHKGIACPTPIKVNENDIFNIKEKPCAIYSFIEGS-QIQSLN----NDAMVSLAKNISTIHHIGAQSKLSRENDMLLPSWKYIINKFKDYDGENISE-FNYIKNLISSLESQFPQ-NLKKSLIHGDLFKDNIFFKNDEVSGFIDFFFTCSDTIVYDLATLINAWFLNYENFNENYFEIFFDHYFGSISWSNEEKESFNFYLKASAIRFFLTRIHDKNFNKDGEVNHKDPMEFFAILRFHEKNNLQDFF 3DXQ Chain:B ((2-257)) -------MTDEARAKLAAIPMLAGYTGPLERLGGLTNLVFRA-----GDLCLRIP-------NRANEAVAAREAAKAGVS-PEVLHVDP-------ATGVMVTRYIAGAQTMSPEKFKTRPGSPARAGEAFRKLHGSGAVFPFRFELFAMIDDYLKVLSTKNV----TLPAGYHDVVREAGGVRSALAAHPLPLAACHCDPLCENFLDTG-ERMWIVDWEYSGMNDPLWDLGDLSVEGK-----FNANQDEELMRAYFGGEA-RPAERGRVVIYKAMCDLLWTLWG------------------------------------

General information: TITO was launched using: RESULT: Template: 3DXQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1091 -18660 -17.10 -78.40 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.65 3D Compatibility (PKB) : -17.10 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.325

(partial model without unconserved sides chains): PDB file : Tito_3DXQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3dxq-query.scw PDB file : Tito_Scwrl_3DXQ.pdb: