Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MASAARPIRDYLPGLLVLAGGLYTVYYYYMYWIRVYGALCLPGYGDPVKVEVTQGIWIRLDAILFPTVFGVILALLGVMLVWKKKFGAAVVALVLSATVINWPAFIMIAGGALLGLLFWRKTLLPTIDLPDETMEWVDAEAGL-SVLKSFRRLPSERLAYSFHNEGG-GRFVVKETSSA----ESAERETRVLNAAAENGLPAPEAKATDG-RFLLMPQLDGF-PFLQPLDFEAWTDEIAGILTTTHSAPKPDMKARRRPAEPVIPDRAF---------DRANWERAARIFREET--ADAVPVFVHGDFEPANVLFSGGRATGLTGWDHAGSGPAQLDVGRMRVALELIHGGNVADRFLAKYKNLSKDRSFRYSRYWDLLAVYGWLEQDPGSIASHWRRFGITTLSEDEVMRRLERFVKTLTDKE 4R7B Chain:A ((18-273)) -----------------------------------------------------------------------------------------------------------------------------GSHMEKIIKEKISSLLSQEEEVLSVEQL-GGMTNQNYLAKTTNKQYIVKFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDST-SIKTKFDKIAPILQTIHTSAKELRGEF-AP-FEEIKKYESLIEEQIPYANYESVRNAVFSLEKRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLES--EFTSQEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSL--------------------------------------

General information: TITO was launched using: RESULT: Template: 4R7B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 976 43420 44.49 183.21 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.52 3D Compatibility (PKB) : 44.49 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.52 QMean score : 0.229

(partial model without unconserved sides chains): PDB file : Tito_4R7B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4r7b-query.scw PDB file : Tito_Scwrl_4R7B.pdb: