Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTVVAALDRLAPRLSPT-A-TRAINARRLSGGASLETWAFDLDDGAPLILRRRTSGSALRETALPLDHEASLIRAASA-VGAPAPSVVHVCAPEDDLGE-AYVMRRLEGETLGRRIVRDEAFAAVRPGLARRCGEVLARIHAVPTEGLPEL--ATSDARGELARYEAIYRELG-A-----ERPIMEAAF-RWLEAAAPSPPERPVLVHGDFRNGNLMIHP---KDGLVGVLDWELAHLGDPAEDLGWICVNSWRFGEWRKPVGGFGDYADLLAGY-G-D--NIPLERVRFWQALGSLKWGVMCLMMYQSFATGADRSVERAMIGRRASETEIDLVALMEAAA 3TDW Chain:A ((5-278)) ------KLHYTTMIMTQFPDISIQSVESLGEGFRNYAILVN----GDWVFRFPKSQQ----GADELNKEIQLLPLLVGCVKVNIPQYVYIGKRS--DGNPFVGYRKVQGQILGEDGMA-VLPDDAKDRLALQLAEFMNELSAFPVETAISAGVPVTNLKNKILLLSEAVEDQVFPLLDESLRDYLTLRFQSYMTHPVYT-RYTPRLIHGDLSPDHFLTNLNSRQTPLTGIIDFGDAAISDPDYDYVYLLEDC---GE---------LFTRQVMAYRGEVDLDTHIRKVSLFVTFDQVSYLLEGL--------------------------------------

General information: TITO was launched using: RESULT: Template: 3TDW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1084 -13798 -12.73 -54.32 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -12.73 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.341

(partial model without unconserved sides chains): PDB file : Tito_3TDW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3tdw-query.scw PDB file : Tito_Scwrl_3TDW.pdb: