Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNNWSFGIDNDKLIGLVLEGKKTATSSLYNFDKIPVIGKESIIHFDNEKDACIVETVDYKIIKYNEMTEELAKLEGEGDLSLNYWKQVHLNFFKSVNPNFKEDDKIIFEIFKVTKNLVEERLKLGKSIASKNTDLLGNIVKVEEINSGFNNTLFNINDKYVIKVCTNKELENTFENEKDFYLSNKD----NCFIPKLYKYDDSKVDCDYIYEVIEKIKGKTLYY-YWYKMNEADREKTIEKLIDIIKKFHSVNGKEY------DWKNKIKGEIKNRIIKCKKYFKIKDYNMILESIDKYDEY-LSDNKFALIHNDLHFDNVIYNN-GKL-TIIDFNDSIQAPIDFEFRLLYMCQE--QPWKWADIEMDPYQKPEDYKNIWNYIKKYYKELNEIKFLEQRMIIYRIWNDTGHLKKYHNVELTKGIVENSKKLI 5IQI Chain:C ((19-257)) --------------------------------------------------------------------------------------------------------------------------------IEHYFDNF-KVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGFAKEKAIYNFLNTNLETNVKIPNIEYSYISD---ELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLE-EYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGN------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5IQI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 863 1325 1.53 5.94 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain C : 0.62 3D Compatibility (PKB) : 1.53 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.219

(partial model without unconserved sides chains): PDB file : Tito_5IQI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5iqi-query.scw PDB file : Tito_Scwrl_5IQI.pdb: