Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQLDSPGTLKQCLISQRPLQGGLTHDNTLLICADGSQWVCKRSLGRH-QSGLATEANVYSLLVN-TRFALPIKLVHLDIASQMLLRPYIEGTTTTSWA-------------QLSIEQAILLLKAIHQITATHHGKVSSPTSLTLNPANQLRVIWRYFRSRTSQRAKM-MDIYGKLSTILQNRQSLFNTLSTFTLNHGDFHPGNLIATPAQQLVPIDWERAHFGDPAFDLALINWHGQDPIVNSALQARAIALYTQCPAEQVALQKRVVCWSLVRLFNDYLYLTSNGLKVDKLAHFEETTAMLLNAAS 5UXD Chain:A ((23-251)) ------------GGTLRTEEIGLDFRVAFARAHDGGDWVLRLPRRPDVLERAAVEGRLLAMLAPHLDVAVPDWRIS---TSELIAYPLLPGSPGLTVAADGEVSWHVDMASTVYARSLGSVVAQLHAVDAEAAAATGIEV---RSP-AQVRGAWRQDLARVGAEFEIAPALRERWEAWLADDG---CWPGHSVLTHGELYPAHTLVEDERITAVLDWTTAAVGDPAKDLMFHQVSAP-----SAIFEVALQAYAEG---------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5UXD.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 815 -31239 -38.33 -146.66 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -38.33 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.183

(partial model without unconserved sides chains): PDB file : Tito_5UXD.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5uxd-query.scw PDB file : Tito_Scwrl_5UXD.pdb: