Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MWQAVNRLLSECLGPAEIRERTELPGGDIHEAWRLSYG-EKEVFVKCNTREM-LPIFTAESDQLSLLARSKTVQVPEVYGVGSD-RDYS----FLLLEYIPLKPLDA--------HNAYCLGQQLAHLHQWSEQLQFGLDFDNDLATTPQPNSWQRRWAQFFAEKRIGWQLQLAAEKGMSFGDIDDITNAVQERLQS---HQPQPSLLHGDLWPANCAASS--NGPVIFDPA-CYWGDRECDLSMLPLY--PTLPAQIYDGYQSVWPLP-IGFIER----QSIYQLYYLLNRSNLFGGKHLINAQKAVDNLLHPEQFSL 5IGH Chain:A ((10-297)) --SQLYALAAR-HGLKLHGPLTVNELGLDYRIVIATVDDGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTAMVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISA-AVDAG--ML--IRTPTQARQKVADDVDRVRRE--------FV----VNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVTYALFALDSGNEEYLAAAKAQL-----------

General information: TITO was launched using: RESULT: Template: 5IGH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1127 -46195 -40.99 -177.67 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -40.99 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.227

(partial model without unconserved sides chains): PDB file : Tito_5IGH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igh-query.scw PDB file : Tito_Scwrl_5IGH.pdb: