Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGLRKTYAKPYSRGISSPLEALRISPVVLSALKGLLSP-D-----TLSRLTPL-GGFEARVYTD---G----------ERVYKVYQPGEAHLAALEAERM---ARAGLGSFVLGVAQVEGQGILVIRHFPGKPFSPESF-TPKALAALSHLFLSLHRLPEPGYVEREELSERLERFAESLGSVP-------E--A---LSLIKALMREV-TW--VAGVEKRFCHRDAWAGNLLLKTQENPSHDDPEVLLVDWVRSGGDDPARDLALLKTGS-LDLL-----------GEKRAREALFRLVQFYPK--E-----------------VRERLAFYVPLTYLHDLHWFRTKKP----E-GFLEALADKLPRALSFFQDCFPLRNRAC 5FTG Chain:A ((9-374)) ------------------------RRRAYLWCKEFLPGAWRGLREDEFHISVIRGGLSNMLFQCSLPDTTATLGDEPRKVLLRLYG----EAMVLESVMFAILAERSLGPKLYGIFPQ----GRLEQFIPSRRLDTEELSLPDISAEIAEKMATFHGMKMPFNKEPKWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLESTPSPVVFCHNDCQEGNILLLEGRE-NSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQAKISSIEFGYMDYAQARFDAYFHQKR----------

General information: TITO was launched using: RESULT: Template: 5FTG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 998 -22283 -22.33 -83.77 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -22.33 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.397

(partial model without unconserved sides chains): PDB file : Tito_5FTG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ftg-query.scw PDB file : Tito_Scwrl_5FTG.pdb: