Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRDMMNKPDLFRSGITSLRDSAKIEQIHKGYSSDSKYIVYGQS-GRPQYILRAYGIDQ-DANKRLEFNRLSMMEEQGVACSRPVDIGVLPDKRLGYMIVSYVEGNEASEELPLLTENEQYAIGVQAGEELKKIHQVACPDSMK-----------SWQDRMAAKHQRYRTEYASCG------------VAIDHEEKLLTFIDSHLHLM--QDRPNLFQHDDYHIGNLIVKD-GKLSGVIDFNRSDWGDPLHDFVKVGMFSA--------EVSVPFAVGQIRGYHHHSEP--DEAFWRLYSLYLGMTLISSVVWILKVRPEELDSMMMKIDRVMDDHDHFELIVPKWYSRFQHEA 2QG7 Chain:D ((109-431)) -------------------DSLEFQIINGG-TNI-LIKVKDMSKQ-AKYLIRLYGPKTD--NREREKKISCILYNKNIA-KKIYVFFTN---G---RIEEFMDGYALSRED-----IKNPKFQKLIAKNLKLLHDIKLNENLYKELQVTQKVPGTRPSFLWNTIWKYFHLLNEERKKICSFDAKANILKLIDFDVLRDSIVEVESLCKRENSPIVLCHCDLLSSNIINTVG-DSISFIDFEYSCPMERAYDIANHFNEYAGFNCDWDLTPSKEEEYHFIMHYLGTDDEELINQLIREIQPFYICSHINWGLWSLLQGM-----------------------------------

General information: TITO was launched using: RESULT: Template: 2QG7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 934 26082 27.93 107.33 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain D : 0.60 3D Compatibility (PKB) : 27.93 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.266

(partial model without unconserved sides chains): PDB file : Tito_2QG7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2qg7-query.scw PDB file : Tito_Scwrl_2QG7.pdb: