Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIDVIISTSRNPRHAR-SKPAFPVVYSTLSPQALVEQVLSNYSIEPITNCLLWNRGL-SDVYLVETEQEYYVLRVSHHHWRS-RLDIEFELELLDFLYQNSLPVAYPLRTRADDLFVTVEALEGDRYAALFPYAKGTVPLGDLTIAQSEIFGRSLAKLHQIGT--KFRPSHHSLILEKLTNKTLS---LEYLLDHSLTTIAPFLEERPEDLKYLRDNITQIKAELKILPRQSPLWTACWGDPHSGNIHFTEDNQITLFDFDQCGYGWRAFDIAKFLQVSLRAGMNRKIRDAFFEGYQTVQKLTSEEEDCLQALTQTAHIWAWAININASVIHCWSRLDYRYFSKRLETLKRLNSPDWQLF 3JR1 Chain:A ((14-309)) ----------NLYFQGMWKSISQVLAEQFGAYYFIKHKEKL------------YSGEMNEIWLINDEVQTVFVKINERSYRSMFRAEADQLALLAKTNSINVPLVYGIGNSQGHSFLLLEAL----------------NKSKNKQSSFTIFAEKIAQLHQIQGPDKYGLDFDTWLGPIYQPNDWQTSWAKFFSENRIGWQLQICKEKGLIFGNIDLIVQIVADTL---SKHNPKPSILHGNLWIENCIQV-DDKIFVCNPA-CYWGDRECDIAFS-------SLFEPFPTNFYQRYNEIYPLEEGYLE-RKLIYQLYYLLNFSYRYYNK--------KQSYVSLTQKLINQILHK-----

General information: TITO was launched using: RESULT: Template: 3JR1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1426 -3384 -2.37 -11.75 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -2.37 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.357

(partial model without unconserved sides chains): PDB file : Tito_3JR1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3jr1-query.scw PDB file : Tito_Scwrl_3JR1.pdb: