Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTAVEHILGEDWTVVPA-GGSTGTAYYAKSD-QRRLFLKRNSSP-----------FLAVLSAQ-GI-VPKLVWTKRLENGDVL----TAQQWLDGKALNP------EEMQHPRVAKLLSKIHHSSELLDMLLRIEKQTQMPTQILAKMKEKVKTT--DDADCSLVIHKALKYLEDRLPYVYYSKPVVCHCDLVHHNWMLSKN-GELYLVDWDNAKVGDPAMDIGRILQSYIPKQEWDQWLSHYGEENN---AHFMQRMHWYLMIDAIHGYTWNVRRDKMKVAHQHMSELQQLLDQVNDWNL 5IGH Chain:A ((19-283)) -----HGLKLHGPLTVNELGLDYRIVIATVDDGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTAMVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDA--GMLIRTPTQARQKVADDVDRVRREFVVNDKRLHRWQRWLDDDS--SWPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMAAHLMVFGEE-GLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVTYALFALD--------------------------

General information: TITO was launched using: RESULT: Template: 5IGH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 932 -1290 -1.38 -5.51 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -1.38 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.324

(partial model without unconserved sides chains): PDB file : Tito_5IGH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igh-query.scw PDB file : Tito_Scwrl_5IGH.pdb: