Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNDAQGSLVHELK-SLPCWPAPVQAIRPL-TGLSGGSLLIELCDGRRYVARSQNAQLQMQAVSRERERQVLCAIGEQVARSD---FLPQVLFYNARWLVVSWLEGSPWPHTLA-------------DDACAQRHLVTLMRRFHPVDSE-LQ-LD-----IPSRLQFYRQQLSAEKQ-PEALFSLCARFASLR---SPCWWFPVLAHHDIHPGNVVSDG-AT-LALIDWEYAARSPLACELILLFEANGFNRAQRQQFCRYYAEAIYSTETVYAADSKCVGSSAAALEDWLHRLQQDIASWQPWCEMLMAMWSAIRFEQTDDVQYAVWRDNYLKAAEQSLGKRE 5UXA Chain:A ((2-299)) -SKDIKQVIEIAKKHNLFL--KEETIQFNESGLDFQAVFAQDNNGIDWVLRLPRREDV--MPRTKVEKQALDLV----NKYAISFQAPNWIIYTEELIAYKKLDGVPAGTIDHNIGNYIWEIDINNVPELFHKSLGRVLAELHSIPSNKAAALDLVVHTPEEARMSMKQRMDAVRAKFGVGENLWNRWQAWLNDDDMWPKKTGLIHGDVHAGHTMIDKDANVTGLIDWTEAKVTDVSHDFIFNYRA--FGEEGLEALILAYKEIGGY--------------------Y-WPKMKEHIIELNAAYPVSIAEFALVS----GIEEYEQMAKEALEV---------

General information: TITO was launched using: RESULT: Template: 5UXA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1146 -21314 -18.60 -79.83 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -18.60 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.335

(partial model without unconserved sides chains): PDB file : Tito_5UXA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5uxa-query.scw PDB file : Tito_Scwrl_5UXA.pdb: