Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTIQQEIFSILGIKGPV--LLGEGSESQTYDYT-----------ADKVVKIYKGPV-----EQDNLDHLCSIYTWLSTHHLPFAVPEIYAIESH---EGTVYLIEKKLAGRPISAVYA---------QLQMYERQRLLSNYLEAVRHLQGVVLPTQPYGSIFHAPGPSPVPYQSWRKFLRETAPLKLPQTQDDLRQEGIDVARLLEKFFQDIENAPDTPEKNFVHGDYFFGNVLVDDELEISAVLDVSWWSVVGDHLMDIAGAVMFLDLYEYVTP----------QEIASLTQQALALYGPEILTAIRIYGVYYSLLLSDCKLLDPAAYSWSIKRLREYMQ 3ATT Chain:A ((23-317)) --------------TVESGVDSTGMSSETIILTARWQQDGRSIQQKLVARVAPAAEDVPVFPTYRLDHQFEVIRLVGEL-TDVPVPRVRWIETTGDVLGTPFFLMDYVEGVVPPDVMPYTFGDNWFADAPAERQRQLQDATVAALATLHSIPNAQNTFSFLT---------DTTLHRHF-NWVRSWYDFAVEGI-GRSPLLERTFEWLQSHWPDDAAAREPVLLWGDARVGNVLYRDF-QPVAVLDWEM-VALGPRELDVAWMIFAHRVFQELAGLATLPGLPEVMREDDVRATYQALTGVE-LGDLHWFYVYSGVMWA----------------------

General information: TITO was launched using: RESULT: Template: 3ATT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1019 -13221 -12.97 -52.88 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -12.97 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.361

(partial model without unconserved sides chains): PDB file : Tito_3ATT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3att-query.scw PDB file : Tito_Scwrl_3ATT.pdb: