Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----MLDQQQIIAVAEAVLTQRYGGQ---------------QHLTE--PEELSGASGTVVLRLRVANNPF-------LSYRSVVVKYSPQTDDPIDDAAF----LREVV-AYQFTTSLS---EEARPGPVMLGYDLEQHAIILTDSGD---------------GDTLATLLENSDEEQRIRLL---RNLGTSLGKMHAG-TAGAELSFNTLLARMVRARDGAREIQAMRESMLENRILDGLEIVRRAGIDVPADVADIAGTVQVHMLHGESRAFTPFDLSPDNIIFADR------------TQFLDYEWAGFRDVIFDVA-----------GVIAGFPQYISARPISE-------------------AETKVFL-------EAWIAEVAGVWPALQ---------------NTETLHSRITAALVGWAFFSVSLLYLDGEEPRGTTRYGGGWIMADNGNYELVQDPEAL------------EEDVERGASVLRS--------PAHTDFTAV--------EKAIRRDIGDAFSALAWYAAAG----DTPRNAVIADFASHVAERLK---------------------------------------------------------- 2QYU Chain:A ((8-627)) ATSSPSSPADWAKKLTDAVLRQKAGETLTAADRDFSNADFRNITFSKILPPSFMERDGDIIKGFNFSNSKFTYSDISHLHFDECRFTYSTLSDVVCSNTKFSNSDMNEVFLQYSITTQQQPSFIDTTLKNTLIRHKANLSGVILNEPDNSSPPSVSGGGNFIRLGDIWLQMPLLWTENAVDGFLNHEHNNGKSILMTIDSLPDKYSQEKVQAMEDLVKSLRGGRLTEACIRPV-ESSLVSVLA---HPPYTQSALISEWLGPVQERFFAHQCQTYNDVPLPAPDTYYQQRILPVLLDSFDRNSAAMTTHSGLFNQVILHCMTGVDCTDGTRQKAAALYEQYLAHPAVSPHIHNGLFGNYDGSPDWTTRAADNFLLLSSQDSDTAMMLSTDTLLTMLNPTPDTAWDNFYLLRAGENVSTAQISPVELFRHDFPVFLAAFNQQATQRRFGELIDIILSTEEHGELNQQFLAATNQKHSTVKLIDDASVSRLATIFDPLLPEGKLSPAHYQHILSAYHLTDATPQKQAETLFCLSTAFARYSSSAIFGTEHDSPPALRGYAEALMQKAWELSPAIFPSSEQFTEWSDRFHGLHGAFTCTSVVADSMQRHARKYFPSVLSSILPLAWA

General information: TITO was launched using: RESULT: Template: 2QYU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2058 44974 21.85 109.69 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 21.85 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.240

(partial model without unconserved sides chains): PDB file : Tito_2QYU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2qyu-query.scw PDB file : Tito_Scwrl_2QYU.pdb: