Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDNRVSSALEDAFPNRTVDNVSSAGISWNAQNRTVAVDFGDGQRVFLKVAVNGD--RSRIERERAVISYVGANCAVSVPNVIATETT----------GPVPYLATEPVSGQPLTTLLADATRTERAALAHDVGSALARVHDCRFEEHGHVVGGDADHLELDTGTWTDVLVE--TISEMREMA-PCDRFDQHFEMVSDAVERNRDRL--DRASATLLHGDPACPNCFRTG----DGVGFLDWELSHVGDPVRDRSRTLDQQFDSLREGSPEGVVSAFYDGYRSQAGGLPSGFEERRPIYEVIRLLSVSGYFERTADYRDESRSDLAAWLEAEMRRRLDAIRR 6EF6 Chain:A ((31-320)) -----AQQALTHYDVSDNASLRLL---NLSENATYLVEDG-EHQSILRVHRQDYHQPHEIESELDWLAALRTDSDVTVPTVVPARDGRRVVTVDPADVPRHVVHFEMVGGAEPDEES--L----TLDDFQTLGRITASLHEHSQRWTRPAG------FGRFSWDWEHCLGDTPRWGRWQDAEGVGASETALLTRAQDLLHRKLEEYGSGPDRYGLIHADLRLANLLVDSSTPQRTITVIDFDDCGFGWYFYDFGTAVSFIEHD---PRLGEWQESWVAGYRSRR-ELPAADEAMLPSFVFLRRLLLLAWMGS-----------------------------

General information: TITO was launched using: RESULT: Template: 6EF6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1201 -9109 -7.58 -34.24 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -7.58 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.240

(partial model without unconserved sides chains): PDB file : Tito_6EF6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-6ef6-query.scw PDB file : Tito_Scwrl_6EF6.pdb: