Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -MNLEQDIRA-ILRTEI--KKHLKFKDVQCELNE----IRS--KNGVYVYRIITTSTSYVIKYFEKNEFRRELDYYNLLIELEIPTIPVISMTDQSIILEDIEQSETYRLGFESDLENENVARGLVAWYTQ----FHKKGRIYLKQNPEQKASLYNEYNELTKNVITDIMTKTGT-----NHYKYWHILINHFDDLIAQSKSYTSTLTYND-FYYTNFIVS----KDESE---------------VIMF-----DYNFLGVGYRYSDVRNIVSSLKGNAKSLFLACYPQCNE-------------GEVILDDVLADLFVLKEA-YKRDQFPSWAKIPVEQLDNGVFEQKVLKLKHYFKFD- 1SRQ Chain:A ((4-339)) KVKLECNPTARIYRKHFLGKEHFNYYSLDTALGHLVFSLKYDVIGDQEHLRLLLRTKCRT---------------YHDVIPISCLFPNVVQMAKL--VCEDV-NVDRFYP-----VLYPKASRLIVTFDEHVISNNFKFGVIYQKLGQTSEEELFS--TNEESPAFVEFLEFLGQKVKLQDFKGF-----RGGLD-VTHGQTGTESVYCNFRNKEIMFHVSTKLPYTEGDAQQLQRKRHIGNDIVAVVFQDENTPFVPDMIASNFLHAYVVVQAEGGPLYKVSVTARDDVPFFGPPLPDPAVFRKGPEFQEFLLTKLINAEYACYKAEKFAKLEERTRAALLETLYEELHIHSQSMMGLGG

General information: TITO was launched using: RESULT: Template: 1SRQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1235 6153 4.98 22.70 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 4.98 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.245

(partial model without unconserved sides chains): PDB file : Tito_1SRQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1srq-query.scw PDB file : Tito_Scwrl_1SRQ.pdb: