Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence METKLGRKIGEGGCSEVYEYGTEQPVKIIKLAKSNTS-MDAMRIEYRNHRTTWE-LGLPVPQPFDLRVV----------DGRPGIVVEQIQGETLMQRFLRGLDQPEVSEDSPIYDDVRMTARILNALHSHSAEA-LP--S--QRESIK------GALLGV--ESLT----APEKKEICAILDDL---PVKQRLCHGDPNPGNIFVRHD---E-GLLIDWMNASAGNPEADLAEYILMVRYAVLPSHLGARIIGRFHSIREMMIDAFMEEYTRLSGVTAEEVDPWMLPMAARKLSADAISDEEKGLLLHEIRVRLNAYKGAEESFRE 6EF6 Chain:A ((47-308)) ------RLL-NLSENATYLVEDGEHQSILRVHRQDYHQPHEIESELDWLAALRTDSDVTVPTVVPARDGRRVVTVDPADVPRHVVHFEMVGGAEPDEES---------LTLDDFQTLGRITASLHEHSQRWTRPAGFGRFSWDWEHCLGDTPRWGRWQDAEGVGASETALLTRAQDLLHRKLEEYGSGPDRYGLIHADLRLANLLVDSSTPQRTITVIDFDDCGFGWYFYDFGTAVSFIEHDP---------------RLGEWQESWVAGYRSRRELPAADEAMLPSFVF-------------------------------------

General information: TITO was launched using: RESULT: Template: 6EF6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 928 -11898 -12.82 -53.35 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -12.82 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.237

(partial model without unconserved sides chains): PDB file : Tito_6EF6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-6ef6-query.scw PDB file : Tito_Scwrl_6EF6.pdb: