Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRDMMNKPDLFRSGITSLRDSAKIEQIHKGYSSDSKYIVYGQSGRPQYILRAYGIDQ-DANKRLEFNRLSMMEEQ--GVACSRPVDIGVLPDKRLGYMIVSYVEGNEASEE-LPLLTENEQYAIGVQAGEELKKIHQVACPDSMKSWQDRMAAKHQ-------RYRTEYASC-GVAID---HEEKLLTFIDSHLHLMQDRPNLFQHDDYHIGNLIVKDGKLSGVIDFNRSDWGDPLHDFVKVGMFS-AEVSVPFAVGQIRGYHHHSEPDEAFWRLYSLYLGMTLISSVVWILKVRPEELDSMMMKIDRVMDDHDHFELIVPKWYSRFQHEA 3HAM Chain:A ((7-278)) ---------EIYEHLNKQIKINELRYLSSGDDSD-TFLC---NE--QYVVKVPKRDSVRISQKREFELYRFLENCKLSYQIPAVVYQ------SDRFNIMKYIKGERITYEQYHKLSEKEKDALAYDEATFLKELHSIEIDCSVSLFSDALVNKKDKFLQDKKLLISILEKEQLLTDEMLEHIETIYENILNNA-VLFKYTPCLVHNDFSANNMIFRNNRLFGVIDFGDFNVGDPDNDFLCLLDCSTDDFGKEFGRKVLKYYQHKA--PEVAERKAELNDVYWSIDQIIYGYERKD-----------------------------------

General information: TITO was launched using: RESULT: Template: 3HAM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1078 -4662 -4.32 -18.21 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -4.32 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.290

(partial model without unconserved sides chains): PDB file : Tito_3HAM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ham-query.scw PDB file : Tito_Scwrl_3HAM.pdb: