Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTAKTPEFSNAQVIELLLQQYKLSGQLKKLPGYCDQNLLLNTDDGKQYIVKIANAVEPKLELEMQNAAMAHLTHKQCAVPCAVNNIQGDNISIITDVSKQSFCLRVLTFIPGKFYADANSNTHSRALWTDLGQFIGNIDLALADFQHPGAFRYLDWDLAQGYRVCMSKKHLLNPQQVNLVDKFLSLYQTQTMPVLSQLPQGVIHNDANDYNLLIDDINTPNKISGLIDFGDMVHSHIVNELAIACAYALMGEKSVHEDILSALTSIVAGYHKIRPLLDVELEVLYSLIALRLCTTVCNAALAIQQQPDNEYLLVSVQPAWQLLEQLSAVNSYAVLCQLRLACQLPVDSGQSAAEIINYRKKHLGKTLSLSYQQPLKMVRGQGAYLFNEQGVPYLDMVN--NVCHVGHCHPKVVAAGQAQLAKLNTNTRYLHDNI--VNYADKLLATMPDKLS-VCMLVNSGSEANELAFRLARCFTKSRELLVVDGAYHGNT-------NACIEASPYKFDGPGGEGAPAYVHKVTLPDPYRGEFLGNNSDAATAYANSVKETIEQLALNNKKPGAFICESLQGVAGQIIMPDGYLTQVYAHVRAAGGVCIADEVQVGFGRVGSHMWAFETQNVIPDIVTLGKPIGNGHPMAAVITTQEIAD---AFVTGMEYFNTFGGNPVSCAIGIAVLDVIEQEQLQNHALTTGQYFQDQLNKLKQRFELIGDVRGKGLFIGVELVEDRTTKQPATDKTSWLIEHFKQHHILLSTEGPFYNILKIKPPLAFNSGDADKFINVLELGLAVLSH 5M46 Chain:A ((27-427)) --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PLAVAGGQGARLVEEDGRELIDLSGAWGAASLGYGHPAIIEAVSRAAANPAGASILSASNAPAVALAERLTASFPGRGTHKVWFGHSGSDANEAAYRAITRATGRTGVIAFIGAYHGCTVGSMAFSGH--------ADG---------LILLPYPDPYRPYQDDPTGDAVLAL---LKERLAAVPAGSIA-AAFI-EPIQSDGGLIVPPDGFLRKFADICRAHGISVVCDEVKVGLARSG-RLHCFEHEGFVPDILVLGKGLGGGLPLSAVIAPAEILDCASAFA-----MQTLHGNPVCAAAGLAVLETIEAENLTTAAERKGKLLREGLARLAERHELIGDIRGRGLACGVELVRNRQSREPARAETAKLIYRAYELGLVLYYVGMNGNVLEMTPPLTMTEDEVRHAVNLLDQAFTELS-

General information: TITO was launched using: RESULT: Template: 5M46.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2300 -6148 -2.67 -16.26 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -2.67 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.245

(partial model without unconserved sides chains): PDB file : Tito_5M46.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5m46-query.scw PDB file : Tito_Scwrl_5M46.pdb: