Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKGWLFLEHLKQRL-EEMKW-------VPLHVECITGAHAGDIFRFEIINHN----GDTEKFIYKEFAEG-----RNNEIEVFKKLENHLQPFTKLV-KWWESNPQAILMVDL-GRPLKDDFMNLPLDSKKKSIVSILQRLSYLHSLKIDQTALTLDAHSITYEWSEWAIDQLKLLNEGHDWASD-----WSKLIESAYQKLKLGQYTVKGPLTLTHGDPHLENVFCYENEIWFIDWEWAAIGSPLRDITILLQDVY-------------------DEELVRYTYRAYKELLDSKK---ISISNDAYRRDFHHLYVDHTTMMLAWEIDKYFQGFTSEARIKEIVEFKMKEINRVVIEEAHISPW 1NW1 Chain:B ((54-398)) -------ERAHMLCARFLGGAWKTVPLEHLRISRIKGGMSNMLFLCRLSEVYPPIRNEPNKVLLRVYFNPETESHLVAESVIFTLLSER-HLG-PKLYGI---FSGGRLEEYIPSRPLSCHEI-----SLAHMSTKIAKRVAKVHQLEVPIWKEPDYLCEALQRWLKQLTGTVDAEHRFDLPEECGVSSVNCLDLARELEFLRAHISLSKSPVTFCHNDLQEGNILLPK-RLVLIDFEYASYNYRAFDFANHFIEWTIDYDIDEAPFYKIQTENFPENDQMLEFFLNYLREQGNTRENELYKKSEDLVQETLPFVPVSHFFWGVWGLLQVELS-------------------------------

General information: TITO was launched using: RESULT: Template: 1NW1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1110 10129 9.12 37.65 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain B : 0.60 3D Compatibility (PKB) : 9.12 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.313

(partial model without unconserved sides chains): PDB file : Tito_1NW1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1nw1-query.scw PDB file : Tito_Scwrl_1NW1.pdb: