Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIPSMCRLMPVSFRIRIYHFLSSLGSRV---YGSSCSLRVQQLPFGVYLKTKSVEDHQALKIEFGALQLVRSQTQIPVPRPLDLLSDTETSYLLTTTLPGQRFGSYIDILSDHDLDLFTRDMQRYLTQLRSISRPRTPKYEIRNAIGGPYYDYRIIAAHDYDKECGDFFGPCTDEE--DFNNILRTPALP----------------YVFHST------GHNIVF---THSDINMRNVLMHNGRISGIADRENSGW-FSDHWEYTKAHYVTKLHKRWLAVVNRIFESFGDFTLDLEIERRLWEYCF 4HS9 Chain:A ((21-307)) --------MSTKYPIVLVHGLAGFSEIVGFPYFYGIADALTQDGHQVFTASLSAFNSNEVRGKQ-LWQFVQTILQETQTKKVNFIGHSQ-GPLACRYVAANYPDSVASVTSINGVNHGSEIADLYRRIIRKDSIPEYIVEKVLNAFGTIISTFS--GHRGDPQDAIAALESLTTEQVTEFNNKYPQ-ALPKTPCGEGDEIVNGVHYYCFGSYIQELIAGENGNLLDPTHAAMRVLNTLFTEKQNDGLVGRCSMRLGKLIKDDYAQDHFDMVNQVAGLVSYNENIVAIYTLHAKYLASKQL-----

General information: TITO was launched using: RESULT: Template: 4HS9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1383 -27714 -20.04 -108.26 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -20.04 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.086

(partial model without unconserved sides chains): PDB file : Tito_4HS9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4hs9-query.scw PDB file : Tito_Scwrl_4HS9.pdb: