Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAACPELVDATFAILASGWHSTAVEVGGRWVFKFPRGAEAEAALLREAAVLRLVRPAVSLPVPAPQVFAGPP------LFSRHEKLPGGYLLGEDYRRLDEPARQAVGDALGRFYAELHRLPPARMRAVGALPVRPWESPAAMRRRALPLLPAAWRERAERLVREYAKLPADPLGS--IFGFF------DGHGWNMAFDSEAGRLNGVYDFADAGIGPLHQEFIYSAFIDADLTERIVAAYERFSGRRLERRRIALLTAAHRLSELAELADEPRHLPDMLAGALDGLALAEAQGLA 4DFB Chain:A ((39-254)) ----PDFTINTIEISGEGNDCIAYEINRDFIFKFPKHSRGSTNLFNEVNILKRIHNKLPLPIPEV-VFTGMPSETYQMSFAGFTKIKGVPLTPLLLNNLPKQSQNQAAKDLARFLSELHSINISGFKSNLVLDFR--EKINEDNKKIKKLLSRELKGPQMKKVDDFYR---DILENEIYFKYYPCLIHNDFSSDHILFDTEKNTICGIIDFGDAAISDPDNDFI------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4DFB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 687 7161 10.42 35.81 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 10.42 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.282

(partial model without unconserved sides chains): PDB file : Tito_4DFB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4dfb-query.scw PDB file : Tito_Scwrl_4DFB.pdb: