Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLAERGRLLLTVVGELLAAEVCAAGTRIRGPDERARRERQRALRIRTALERLGPLYVKAGQVLSTRED-LVPPAVARELESLHDRAAPCPFDRMSAVLEAELGPDWRRRFRHFDTERPLGCASIAQAYAAVLDDGRSVVVKVQRPGIAAQMTTDMRLLRGMTRTLARRTPVLSETIDFEAMLAVIFEAVRNELDLTLEAANMDEARAAVTAVPGIDVPGVVH--ATERVLIQRRAPGTSVRDADPAAFDDAERERVGTGLLTVMYQGYFIDHRFHADPHPGNVFVCPGG-PTTLIDWGMIGRLDRHLSSTLLMTLLGLANNDAHAVARAWSEMGRPTPWADIGAFEQDMSVVVPRLASIPLDRLRFGASLATLLRLSTRRGIRTNPMIGLLGKSFANMEGTVRHLAPHLSATDVLVRQTGRVLGEYAAQSLSQQQLGFTALQLLSGLETLLPHARAVGRSLSGGDLTLQHTAVTRPFSLVDHRSSARIRRAEHHLLGAAALVWLLRRRGRG 5I35 Chain:A ((21-283)) --------------------------------------------------------LKLGQMLSIQDDAFINPHLAKIFERVRQSADFMPLKQMMKTLNNDLGPNWRDKLEYFE-ERPFAAASIGQVHLARMKGGREVAMKIQYPGVAQSINSDVNNLMAVLNM----SNMLPEGLFPEHLIDVLRRELALECDYQREAACARKFRDLLKGHPFFYVPEIVDELCSPHVLTTELVSGFPLDQAE--GLSQEIRNEICYNILVLCLRELFEFHFMQTDPNWSNFFYDPQQHKVALLDFGATREYDRSFTDLYIQIIRAAADRDRETV-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5I35.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 986 7097 7.20 27.40 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 7.20 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.218

(partial model without unconserved sides chains): PDB file : Tito_5I35.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5i35-query.scw PDB file : Tito_Scwrl_5I35.pdb: