Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSGWCADAPALIARLASQWELSLGESLPDGASSIALRCRLPDGTPAVLKVSPDRALMTEQVDMLGWFAASGRVPSVLAVDEESGAMVLEEIVPGTPAGDMPVAP----LPGQWSDLLAALHGVAPPPLPTRVLRGRCEEAFARVGRRLAEPAISARMDVTAWDRAIQRCERLLNTEAT----TVLLHGDLHLGNVLDGGAQRVLMAID-PKACVGDPCFDAVDYVVAGAGLEGIETRCEQVAAACGLDGDRL-HAWSQVIAPFAAIAH----LGSGGKEPLIDELLESAR 5IGT Chain:A ((98-288)) --------------------------------------------------------------------------------EDSTAMVIQ---PGSSTPDWVVPQDSEVFAESFATALAALHAV---PISAAVDAGMLIRTPTQARQKVADDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMAAHLMV-FGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLA-FGAVTYALFALDSGNEE-----------

General information: TITO was launched using: RESULT: Template: 5IGT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 737 -40886 -55.48 -230.99 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -55.48 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.217

(partial model without unconserved sides chains): PDB file : Tito_5IGT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igt-query.scw PDB file : Tito_Scwrl_5IGT.pdb: