Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTLSHLVDVVRRAHPDVDLEGAGVHSGQFHDVL---IARDRVFRFPKTAGAAAELPGRVAVLTAVDAVELGVGVPVPLSEV-RDGGPH-----GFLVLSRLHGTPLERGDATS--PEVIDVVAAEFARVLRAMAGADVE--KLRLVLPVADAGRWRGFAGRVRATLFPLMSEDGRARAERE--------LAAAVAMDHVATGLVHGDLGGENVLWQQVEELPRLTGIVDWDEAKVGDPAEDLAAV---GASYGPELVERVVALLGAGDL---WPRIRAYQGTFALQQALAGAEDGDDEELEDGLTAYR 4DFU Chain:A ((25-317)) YTFDQVEKAIEQLYPDFTINTIEI-SGEGNDCIAYEINRDFIFKFPKHSRGSTNLFNEVNILKRIHN-----KLPLPIPEVVFTGMPSETYQMSFAGFTKIKGVPLTPLLLNNLPKQSQNQAAKDLARFLSELHSINISGFKSNLVLD------FREKINEDNKKIKKLLSRELKGPQMKKVDDFYRDILENEIYFKYYPC-LIHNDFSSDHILFDT--EKNTICGIIDFGDAAISDPDNDFISLMEDDEEYGMEFVSKILNHYKHKDIPTVLEKYRMKEKYWSFEKIIYGKEYGYMDWYEEGLNEIR

General information: TITO was launched using: RESULT: Template: 4DFU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1198 23117 19.30 86.90 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.74 3D Compatibility (PKB) : 19.30 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.396

(partial model without unconserved sides chains): PDB file : Tito_4DFU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4dfu-query.scw PDB file : Tito_Scwrl_4DFU.pdb: