Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MVDASEGSSKEEEAGHVPHVEVESARNCGEAWTKTRSSPFTSVWHISGETGAAAGHCREGSLHKLIATNNISGVRLWLSNPFCRSMINDLNHAGETPLHVAVQCCSPRILSLLLDPPPLQISPSLLASCSLRLPRPLAGTAEPPSGAPASSNEGAPLGGSAEEVADAGVASLKTQQPFSAETAISSVVATDAAAACDQLAMAAAARVDFSLSVDGIPTLHLLIGRTAFGGARGEDAMECLKRILLHIGRFQPGAADRCPRCSPGKTKPTACGRERSGVKAESVAFECGDHVSDPQAGGLRESQISHPGLGGQQEYGCQCCGGTLGSLDSRRKKEMREARDRVCCPEVFTVERQTSAADSVMLDLEEMDLQGRTALHLACSFGVSPVAELLLHAGASPWARRLGLGQLPIHSAIDADDPACCLLLLRHTLPCRFFVNFSCICGRRCDSGATGGRQAPVNTLALKRSPAATKFVFQLVRRCVRRGAWRCFRALLLYPTESSACFSGSVEGGDKAEGLQPGGSNSAASRRCEVENLGRKEDACERQGGRVRCVTKDVAASWCSGLHGLLDGEVRAWLYLREEARRAGALLDLKEQLQRVVTTSPACCQAAAASWTAVPASPCDGHKFGVKSESETGLLLSLFGTHGDAEGKTAKRGEGRTMVVTHALCLEHLPLPEPADMPLKRHKLMQRFPENPSRLEVVTSD--RCGILRTREFSPLLWMDDPMPASLSDILRVHSMSYIARLKLRVEDAFGISTRLPSLSPPLSYAPGQTAAAATATGAEALAAAAAALDSKLQKEQYEKQKRQQIENVGSAGRYSFVFADGDTPVTCFSWAAAVHAAGAVIAAVDAVCERKCRNAFCAVRPPGHHLGNWGAAQTASNQLTDEDIAAGSQGFCLLNNVAIGAAYAKYNYARKGIRRIAIVDFDVHHGNGTEQIIRNVGMKIRKVKQPQGAALRLYRRAADELRGTSTLVSSNLSRDAPAAGDNSAHCGPDPCEVDATGSEKTPSQGYHVGDGGKVSAALPPQLAGPVDVPQWMGWRDERDAEELFFASIHAFD-GTFYPGTGA--DCEDF-SGPVVINVNILPHPTGPSLHKCCRCQKCSGRPCAGGAHRRCPRGRDAGVSYANASPMEVEMAARTPDVPVQVSWSCHFCRHPTLAPSSVAARRLFLKRIIRPLLRFGPDLLFISAGFDGHAQDQIGGAFGGFAEEDFRFFTHALVRCAERTCEGRVVSVLEGGYSVSGGVSSTLAASVKEHLRGLMRTPAGGSCFAGALGSEQPGNEAHEAAPRESSLSFPGKNGWEEDIEVGDAATSDVTASDVEESWQLIEGENTGMDGADSSDALLDEGSRRNGSDDGPSADALSHRRPREAKTLDGCASPGPHSSTETASEASGHCEREHSADFPPGADACRSRGTEHRSPTNAFASSASAPSAQRYGDSATVNNNDLLTLRALCRGTDGCLR 5TD7 Chain:A ((4-415)) -----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ASGSALIFDEEMSRYKLLWTD----------PACEIEVPERLTVSYEALRTHGLAQRC------KAVPVRQATEQEILLAHSEEYLEAVKQTPGMN---------------------------------------------VEE---L-----------MAF--SKKYNDVYFHQNIYHCAKLAAGATLQLVDSVMKREVRNGMALVRPPGHHSQR-----------------SAANGFCVFNNVAFAALYAKKNY---NLNRILIVDWDVHHGQGIQYCFEED--------------------------------------------------------------------------------------------------------PSVLYFSWHRYEHQSFWPNLPESDYSSVGKGKGSGFNINLPWNK--------------------------------VGMT-------------------------------------NSDYLAAFFHVLLPVAYEFDPELVIVSAGFDSAIGDPEGE--MCALPEIFAHLTHLLMPLA----AGKMCVVLEGGFNLTS-----LGQSVCQTVHSLLGDPTPRISG-----LGTACDSALESIQN---VRNVQSSYWSSFKHLA----------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5TD7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2290 -20700 -9.04 -58.15 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -9.04 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.095

(partial model without unconserved sides chains): PDB file : Tito_5TD7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5td7-query.scw PDB file : Tito_Scwrl_5TD7.pdb: