Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRRTGAPAQADSRGRGRARGGCPGGEATLSQPPPRGGTRGQEPQMKETIMNQEKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKK-LQFSLKKLGVN-NIS-GIEEVNMFTNQGTVIHFNNPKVQASLAANTFTITGHAETKQLTEMLPSILNQLGADSLTS-LRRLAEALPKQS-VDGKAPLA-TGEDDDDEVP--DLVENFDEASKNEAN 3NKU Chain:A ((12-205)) -----GSLYSDERDK-----------PLLS-----------PTAQKKFEEYQNKLANLSKIIREN-EGNEVSPWQEWENGLRQIYKEMIYDAFDALGVEMPKDMEVHFAGSSDLDAFVI-VKNDEDIKKVKPVFDALNNLCQRIFTASINPSRLIG-TPDDLFGML--------KDGMVADVEATAMSILTSKPVLPRYELGEELRDKIKQEPS

General information: TITO was launched using: RESULT: Template: 3NKU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 675 39014 57.80 232.22 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 57.80 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.316

(partial model without unconserved sides chains): PDB file : Tito_3NKU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3nku-query.scw PDB file : Tito_Scwrl_3NKU.pdb: