Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAAPAQSEQMRRLAGLLG-VKLDPFSTGGESLTFGIT---GTELLVTIPTEPG-EQDVQRQAELTRRVADRISIPVPEIVRVVAE-------AEAVVVRRLPGVPLIAVPTPQRAALTRSVALAVGTVLAELHTWDRDGYADLA-P--VDEYSPEEWRAETAELVSDLTPLLTTDQRDDVRRFLGRPAPRPAGRHVLSHNDLGIEHILVSP-DAVTGVIDWNDAAITDPAYDFGLLLRDLGPEALRTALARYAHQIGG-DPELLERASYYAVCALLEDLAFGHETGRVEYVAKSLHGWRWTFQAAG 5IGH Chain:A ((8-297)) --DTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVDDGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTAMVIQPGSSTPDWV-VP--QDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVADDVDRVRREF-V-VNDKRLHRWQRWLDDDS-SWPDFSVVVHGDLYVGHVLIDNTERVSGMIDWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVTYALFALDSGNEEYLAAAKAQL--------

General information: TITO was launched using: RESULT: Template: 5IGH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1216 -1409 -1.16 -5.16 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -1.16 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.469

(partial model without unconserved sides chains): PDB file : Tito_5IGH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5igh-query.scw PDB file : Tito_Scwrl_5IGH.pdb: