Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHVPLSIHTTSYSMAAWDITTADEAWLIELCHKSADEGRQIGGQEHGGPKVVRIS-----DRIVAKYG-NIR-RS---ELAAQEVAYHNTDRSIVHIPKVHRFFESDGQSYLFMEYVEGQTLEDVDFET-------HKDIPIRVANILAHLQQILGDCDSPGPVTGGEAHGYIFGDEGAGTAFDSIEDMNVYMNKRLDKMNEYLSRQEDQRRFDHLDLTPYALVLCHGDICRRNIIFESDGSLCLVDWGFAGFYPRIFELAAISYVVQNNAAFKDPIIHEFTKLLSLTDKEKQDIDRFRAVRFANLRWSFRDRRTTAEEDKFIQELYETQKRIKE-EQETAGIQSFIISELLDGLL--EQKSRPDLEVRHVPRPWTGSQTAIGHVKRVVVVGLDVNVASFILAMKILSSRRIFVIRTTQGSPK 5TVT Chain:A ((18-267)) ------------------------------------------IGTGGFAKVKLACHILTGEMVAIKIMDKNTLGSDLPRIKTEIEALKNL--RHQHICQLYHVLETANKIFMVLEYCPGGELFDYIISQDRLSEEETRVVFRQIVSAVAYV---------------------------------------------------------------------HSQGYAHRDLKPENLLFDEYHKLKLIDFGLCAK------------GSL---AYAAPELIQG----KSYLGSEADVWSMGI-----LLYVLMCGFLPFDDDNVMAL----YKKIMRGKYDVPKWLSPSSILLLQQMLQVDPKKRISMKNL-LNHPWIMQD--------------------------------------------

General information: TITO was launched using: RESULT: Template: 5TVT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1017 19005 18.69 87.58 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.60 3D Compatibility (PKB) : 18.69 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.199

(partial model without unconserved sides chains): PDB file : Tito_5TVT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5tvt-query.scw PDB file : Tito_Scwrl_5TVT.pdb: