Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTIRPDIFSEIKQYIDIFHIEPVRKGYSEDMKYLLTAKSGERFLLRITESDNTQILAGRKEMYSLMDTLAKY---SSLVPRSRACWISHNAYSCIMIHEYIEGDDGEE-SLHRYSQETQYEIGYRAGIELKKLHQLAAPATLS----SWYTRKKQKHERYCDACNREHLAPE-ELDLEPIHRYIADNIHLMKNVSQTFQHDDYHPANLIINN-GQLTGIIDFNRYDWGDPIHDFYKLAFFSR--EISIPFSRGQIDGYFMGNVPSDFWKRYALYNAMSIVPSIVWFSRMASRKEFRDELKRAITLIRTLIDDHKGFTSAIPQWYQ 5IQC Chain:A ((23-289)) -----------FDNFKVDSIEIIGSGYDSV-AYLVN----NEYIFKTKFSTN--KKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISD--ELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVL-EEYILLRETIYN-DLTDIEKDYIESFMERLNAT--TVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYL-LEDSEEEIGTNFGEDILRMYGN-IDIEKAKEYQDIVEEYYPIETIVYGIKNIKQE--------------------------------

General information: TITO was launched using: RESULT: Template: 5IQC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1128 -14956 -13.26 -58.65 target 2D structure prediction score : 0.52 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -13.26 2D Compatibility (Sec. Struct. Predict.) : 0.52 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.216

(partial model without unconserved sides chains): PDB file : Tito_5IQC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5iqc-query.scw PDB file : Tito_Scwrl_5IQC.pdb: