Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLSTIFKRHHL-S-VITYEEITSGFSMTQKYLLTTHEKQYILKIYPLAT--FERIQQQRSFLSQHQK-NHVHCQLPIFYDTL----------EQWCYTISEYLAGKSLDRLLPCLSQEKQYELGMQAGQELQKIHLLACPNLHF------NW--YQARLRKYQQ---KKEQCAKLGLNFHQKTAIETFIDKHFHLL--KESAVCFQHDDFHPQNLLYD-N---QKIIVLDFDSFDWGDPWEEFFKLPKYTV--SVSQAFANGQLMGYFEG-NIPQNFWKKYHLFVALNCYASQIGGYQSGNLASVQLRTKKIIQTHYFSNQPPQWFQQKPTS 6EF6 Chain:A ((34-322)) -----ALTHYDVSDNASLRLL-NLSEN-ATYLVEDGEHQSILRVHRQDYHQPHEIESELDWLAALRTDSDVTVPTVVPARDGRRVVTVDPADVPRHVVHFEMVGGAEPDEES--LT----LDDFQTLGRITASLHEHSQRWTRPAGFGRFSWDWEHCL-GDTPRWGRWQDAEGVGASETALLTRAQDLLHRKLEEYGSGPDRYGLIHADLRLANLLVDSSTPQRTITVIDFDDCGFGWYFYDFGTAVSFIEHDPRLGEWQESWVAGYRSRRELPAADEAMLPSFVFLRRLLLLAWMGSHT--------------------------------

General information: TITO was launched using: RESULT: Template: 6EF6.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1089 4143 3.80 16.50 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 3.80 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.164

(partial model without unconserved sides chains): PDB file : Tito_6EF6.pdb: (Unconserved sides chains are recalculated) : Sequence: align-6ef6-query.scw PDB file : Tito_Scwrl_6EF6.pdb: