Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNQIVEDAGSLDVFRWPSSSPPSPENLEHAARLCQGALGSTS-----VAVVATPYNHAGWSAQDCIKVSFPSE-------NRSFIAKVPRLVDENS-ATACKNEAQRASWASQHQIGPAVIAIDDLSGAFAMEFLSGQTLSAQMMLQDHLKTCTIGLLRRIHDAPSQHYMQ---IYNAPAAVKRMLQAADKER---L--LDGDDGL-LLESLITWTFQKVGCQPTFQTLVPCHNDFHSQNIMLDQQ------GTLWAIDFEDCDLGDPMWDLGYLTANMGLE------------------PL--D-LAGIY-GCNM---------------EQ-KKRLEVFYYLAIGHCAVWSAIHGPLWTQHYRDCMLRLRRGWLRL 5FUT Chain:A ((4-352)) ---------------------PEPRTRRRAYLWCKEFLPGAWRGLREDEFHISVIR-GGLS-NMLFQCSLPDTTATLGDEPRKVLLRLYG------GAEAMVLESVMFAILAERSLGPKLYGIFP--QGRLEQFIPSRRLDTEELSLPDISAEIAEKMATFHGMKMPFNKEPKWLFGTMEKYLKEVLRIKFTEESRIKKLHKLLSYNLPLELENLRSLLE---STPSPVVFCHNDCQEGNILLLEGRENSEKQKLMLIDFEYSSYNYRGFDIGNHFCEWMYDYSYEKYPFFRANIRKYPTKKQQLHFISSYLPAFQNDFENLSTEEKSIIKEEMLLEVNRFALASHFLWGLWSIVQAKIS------------------

General information: TITO was launched using: RESULT: Template: 5FUT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 970 -277 -0.29 -1.07 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -0.29 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.250

(partial model without unconserved sides chains): PDB file : Tito_5FUT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5fut-query.scw PDB file : Tito_Scwrl_5FUT.pdb: